Study on a novel spherical polysaccharide from Fructus Mori with good antioxidant activity

Dong Yu-Hao; Chen Chun; Huang Qiang; Fu Xiong

doi:10.1016/j.carbpol.2020.117516

Study on a novel spherical polysaccharide from Fructus Mori with good antioxidant activity

Carbohydr Polym. 2021 Mar 15:256:117516. doi: 10.1016/j.carbpol.2020.117516. Epub 2020 Dec 16.

Authors

Dong Yu-Hao¹, Chen Chun², Huang Qiang³, Fu Xiong⁴

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
² School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; SCUT-Zhuhai Institute of Modern Industrial Innovation, Zhuhai, 519715, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China. Electronic address: chenc@scut.edu.cn.
³ School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; SCUT-Zhuhai Institute of Modern Industrial Innovation, Zhuhai, 519715, China; Guangzhou Institute of Modern Industrial Technology, Nansha, 511458, China.
⁴ School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China; SCUT-Zhuhai Institute of Modern Industrial Innovation, Zhuhai, 519715, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, 510640, China. Electronic address: lfxfu@scut.edu.cn.

PMID: 33483037
DOI: 10.1016/j.carbpol.2020.117516

Abstract

A novel polysaccharide (MFP1P) was isolated from Fructus Mori, followed by purification via DEAE-52 cellulose and 27 % ethanol fraction. The MFP1P had the molecular weight of 56.78 kDa and the total sugar content of 93.32±0.54 %. And the MFP1P is mainly composed of glucose, galactose, galacturonic acid and mannose with molar ratio of 66.62 %, 13.94 %, 18.24 % and 1.20 %, respectively. MFP1P was mainly composed of →3)-α-D-Gal (1→, β-D-Man-(1→ and →6)-α-D-Glc (1→ glycosidic bond and showed a spherical chain conformation with uniform distribution in solution. The MFP1P exhibited great antioxidant activity with oxygen-free radical absorption capacity (ORAC) values of 291.63±6.81 μmol TE/g and MDA IC₅₀ of 0.289±0.022 mg/mL.

Keywords: Antioxidant activity; Conformation; Fructus Mori; Polysaccharide; Structure.

MeSH terms

Amidines / antagonists & inhibitors
Amidines / chemistry
Animals
Antioxidants / chemistry*
Antioxidants / isolation & purification
Antioxidants / pharmacology
Carbohydrate Sequence
Chemical Fractionation / methods
Complex Mixtures / chemistry
Fruit / chemistry*
Galactose / chemistry
Galactose / isolation & purification
Glucose / chemistry
Glucose / isolation & purification
Hexuronic Acids / chemistry
Hexuronic Acids / isolation & purification
Liver / drug effects*
Liver / metabolism
Malondialdehyde / antagonists & inhibitors
Malondialdehyde / metabolism
Mannose / chemistry
Mannose / isolation & purification
Mice
Molecular Weight
Morus / chemistry*
Oxidants / antagonists & inhibitors*
Oxidants / chemistry
Plant Extracts / chemistry
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Polysaccharides / pharmacology

Substances

Amidines
Antioxidants
Complex Mixtures
Hexuronic Acids
Oxidants
Plant Extracts
Polysaccharides
galacturonic acid
Malondialdehyde
2,2'-azobis(2-amidinopropane)
Glucose
Mannose
Galactose