The mitochondrial genome of Atrocalopteryx melli Ris, 1912 (Zygoptera: Calopterygidae) via Ion Torrent PGM NGS sequencing

Shaolin Xu; Zhaoying Guan; Qi Huang; Lei Xu; Andy Vierstraete; Henri J Dumont; Qiuqi Lin

doi:10.1080/23802359.2017.1413307

The mitochondrial genome of Atrocalopteryx melli Ris, 1912 (Zygoptera: Calopterygidae) via Ion Torrent PGM NGS sequencing

Mitochondrial DNA B Resour. 2018 Jan 11;3(1):115-117. doi: 10.1080/23802359.2017.1413307.

Authors

Shaolin Xu¹, Zhaoying Guan², Qi Huang¹, Lei Xu³, Andy Vierstraete⁴, Henri J Dumont^{1

4}, Qiuqi Lin¹

Affiliations

¹ Institute of Hydrobiology, Jinan University, Guangzhou, China.
² School of Applied Biology, Shenzhen Institute of Technology, Shenzhen, China.
³ South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment; Key Laboratory of South China Sea Fishery Resources Development and Utilization, Ministry of Agriculture, Guangzhou, China.
⁴ Department of Biology, University of Gent, Gent, Belgium.

Abstract

The mitochondrial genome of Atrocalopteryx melli was sequenced and assembled via Next-Generation Sequencing (NGS) and iteratively assembly process with a reference seed. This genome is 15,562 bp long and A + T biased (71%), with 37 genes arranged in common order of Odonata. All protein-coding genes are initiated by typical "ATN" codon, and 9 genes are terminated with a complete stop codon, except nad4, nad5, cox2, and cox3, which are terminated with an incomplete codon "T(aa)". The S5 intergenic spacer is absent in this genome, supporting that lacking of S5 as a specific character for damselflies. The A + T rich region of A. melli is 267 bp longer than that of A. atrata. This mitogenome provides new molecular information for understanding of A. melli and Atrocalopteryx.

Keywords: Atrocalopteryx melli; NGS sequencing; damselflies; intergenic spacer; mitochondrial genome.