First near complete mitochondrial genome of large toothed toad, Oreolalax major (Anura: Megophryidae) from southwest China

Fuwen Liu; Yusong Liu; Yan Li; Qingyong Ni; Yongfang Yao; Huailiang Xu; Mingxian Yang; Taifu Wang; Jiacai Wang; Dingqi Rao; Mingwang Zhang

doi:10.1080/23802359.2016.1144109

First near complete mitochondrial genome of large toothed toad, Oreolalax major (Anura: Megophryidae) from southwest China

Mitochondrial DNA B Resour. 2017 Jan 18;2(1):37-38. doi: 10.1080/23802359.2016.1144109.

Authors

Fuwen Liu^{1

2}, Yusong Liu¹, Yan Li¹, Qingyong Ni¹, Yongfang Yao¹, Huailiang Xu³, Mingxian Yang¹, Taifu Wang⁴, Jiacai Wang⁵, Dingqi Rao⁶, Mingwang Zhang¹

Affiliations

¹ College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
² Chengdu Leique Ecology-Environmental Protection Science and Technology Limited Company, Chengdu, Sichuan, China.
³ College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan, China.
⁴ Sichuan Baodinggou Nature Reserve, Maoxian, Sichuan, China.
⁵ Sichuan Wawushan Nature Reserve, Hongya, Sichuan, China.
⁶ State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.

Abstract

In this study, the near complete mitogenome sequence (15,469 bp) of Oreolalax major was determined using polymerase chain reaction (PCR). It includes 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes and 19 transfer RNA (tRNA) genes (GenBank accession number KU310894). The features of O. major have one more tRNA gene (tRNA^Met ) behind the original one before ND2 which is similar to Leptobrachium boringii. Phylogenetic analyses were based on the concatenated sequences of the 13 protein-encoding genes of O. major and other related species.

Keywords: Mitochondrial genome; Oreolalax major; phylogenetic analysis.