The complete chloroplast genome sequence of a new variety of Dendrobium officinale 'zhong ke IV hao'

Zhimin Zhong; Guifang Zhang; Xiaoping Lai; Song Huang

doi:10.1080/23802359.2016.1219632

The complete chloroplast genome sequence of a new variety of Dendrobium officinale 'zhong ke IV hao'

Mitochondrial DNA B Resour. 2016 Sep 2;1(1):669-670. doi: 10.1080/23802359.2016.1219632.

Authors

Zhimin Zhong¹, Guifang Zhang¹, Xiaoping Lai¹, Song Huang²

Affiliations

¹ School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, China.
² Dongguan Mathematical En-gineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangdong Dongguan, China.

Abstract

Here, we reported and characterized the complete chloroplast (cp) genome sequence of Dendrobium officinale' zhong ke IV hao', a new variety from self-cross plants of imported Sichuan D. officinale, obtained exclusively using Illumina and PacBio sequencing technology. The genome size is 152,185 bp, containing a large single copy (LSC) region (85,094 bp) and a small single copy(SSC) region (14,521 bp) that were separated by two inverted repeat (IRs) regions (26,285 bp).The GC content was 37.46%. In total, the complete cp DNA contains 89 protein-coding genes, 30 tRNA genes, and 8 rRNA genes. Twelve genes contained one or two introns. Phylogenetic analyses showed that the chloroplast genome of D officinale 'zhong ke IV hao' is related to that of the traditional D.officinale.

Keywords: Complete chloroplast genome; Dendrobium officinale ‘zhong ke IV hao’; PacBio; single-molecule real-time (SMRT).