Evaluation of BD Phoenix and Microscan WalkAway for determination of fosfomycin susceptibility in Staphylococcus aureus

Alessandro Bondi; Marco Peradotto; Gabriele Bianco; Davide Ghibaudo; Anna Maria Barbui; Cristina Costa; Rossana Cavallo

doi:10.1016/j.diagmicrobio.2020.115240

Evaluation of BD Phoenix and Microscan WalkAway for determination of fosfomycin susceptibility in Staphylococcus aureus

Diagn Microbiol Infect Dis. 2021 Apr;99(4):115240. doi: 10.1016/j.diagmicrobio.2020.115240. Epub 2020 Oct 17.

Authors

Alessandro Bondi¹, Marco Peradotto², Gabriele Bianco², Davide Ghibaudo², Anna Maria Barbui², Cristina Costa², Rossana Cavallo²

Affiliations

¹ Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, University of Turin, Turin, Italy. Electronic address: abondi.biologo@gmail.com.
² Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, University of Turin, Turin, Italy.

PMID: 33465684
DOI: 10.1016/j.diagmicrobio.2020.115240

Abstract

We evaluate the performance of BD Phoenix (BD Diagnostic; Hunt Valley, MD) and MiscroScan WalkAway (Beckman Coulter Inc; Carlsbad, CA) systems versus reference agar dilution method for fosfomycin susceptibility determination in 200 Staphylococcus aureus clinical isolates. Both methods exhibit ≤89.0% of categorica agreement and ≥63.3% of very major error. All commercial antimicrobial susceptibility systems show poor concordance with reference method.

Keywords: Agar Dilution; Fosfomycin; Methicillin-Resistant Staphylococcus aureus; Staphylococcus aureus.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteriological Techniques*
Drug Resistance, Bacterial*
Fosfomycin / pharmacology*
Staphylococcus aureus / drug effects*

Substances

Anti-Bacterial Agents
Fosfomycin