Protocol for mouse trophoblast stem cell isolation, differentiation, and cytokine detection

STAR Protoc. 2020 Dec 30;2(1):100242. doi: 10.1016/j.xpro.2020.100242. eCollection 2021 Mar 19.

Abstract

Trophoblast cells are the first differentiated cells formed from a fertilized egg during mammalian development, and they secrete several autocrine and paracrine factors essential for sustaining pregnancy. In pathological conditions, these cells secrete various proinflammatory cytokines affecting both maternal and fetal health. Here, we provide a detailed protocol for isolation, maintenance, differentiation, and detection of factors secreted from trophoblast stem (TS) cells. This protocol provides conditions for inducing genotoxic stress in differentiated TS cells and detecting the effects on cytokine production. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).

Keywords: Cell biology; Cell differentiation; Cell isolation; Stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques*
  • Cell Differentiation*
  • Cell Separation*
  • Cytokines / metabolism*
  • Female
  • Mice
  • Pregnancy
  • Stem Cells* / cytology
  • Stem Cells* / metabolism
  • Trophoblasts* / cytology
  • Trophoblasts* / metabolism

Substances

  • Cytokines