Molecular identification and phylogenetic analysis of a Callosciurus notatus complete mitogenome from Peninsular Malaysia

Puteri Nur Syahzanani Jahari; Shahfiz Mohd Azman; Kaviarasu Munian; Nor Hazwani Ahmad Ruzman; Mohd Shahir Shamsir; Stine R Richter; M Thomas P Gilbert; Faezah Mohd Salleh

doi:10.1080/23802359.2020.1797583

Molecular identification and phylogenetic analysis of a Callosciurus notatus complete mitogenome from Peninsular Malaysia

Mitochondrial DNA B Resour. 2020 Aug 26;5(3):3004-3006. doi: 10.1080/23802359.2020.1797583.

Authors

Puteri Nur Syahzanani Jahari¹, Shahfiz Mohd Azman², Kaviarasu Munian², Nor Hazwani Ahmad Ruzman², Mohd Shahir Shamsir³, Stine R Richter⁴, M Thomas P Gilbert⁴, Faezah Mohd Salleh¹

Affiliations

¹ Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
² Forest Biodiversity Division, Forest Research Institute Malaysia, Kepong, Malaysia.
³ Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Higher Education Hub, Muar, Malaysia.
⁴ Section for Evolutionary Genomics, The GLOBE Institute, University of Copenhagen, Copenhagen, Denmark.

Abstract

The mitogenome of a plantain squirrel, Callosciurus notatus, collected from Bukit Tarek Forest Reserve (Extension), Selangor, Malaysia was sequenced using BGISEQ-500RS technology. The 16,582 bp mitogenome consists of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. A phylogenetic and BLASTn analysis against other available datasets showed that the mitogenome matched with 99.49% similarity to a previously published C. notatus mitogenome from Peninsular Malaysia. However, it also diverged by nearly 8% (92.24% match) from a second previously published mitogenome for the same species, sampled in East Kalimantan, Indonesia. This suggests a difference in landscape features between both localities might affect its genetic connectivity.

Keywords: Callosciurus notatus; mitogenome; phylogenetic analysis.