Genetic damage in lymphocytes of lung cancer patients is correlated to the composition of the respiratory tract microbiome

V G Druzhinin; L V Matskova; P S Demenkov; E D Baranova; V P Volobaev; V I Minina; A V Larionov; V A Titov; A Fucic

doi:10.1093/mutage/geab004

Genetic damage in lymphocytes of lung cancer patients is correlated to the composition of the respiratory tract microbiome

Mutagenesis. 2021 May 31;36(2):143-153. doi: 10.1093/mutage/geab004.

Authors

V G Druzhinin¹, L V Matskova^{1

2

3}, P S Demenkov⁴, E D Baranova¹, V P Volobaev¹, V I Minina^{1

5}, A V Larionov¹, V A Titov⁶, A Fucic⁷

Affiliations

¹ Kemerovo State University, Kemerovo, Russian Federation, Krasnaya St., 6.
² Institute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation, Kaliningrad, st. A. Nevsky, 14.
³ Department of Microbiology, Tumor Biology and Cell Biology (MTC), Stockholm, Sweden, 171 65, Solna, Solnavägen, 9.
⁴ Institute of Cytology and Genetics SB RAS, Novosibirsk, Russian Federation, Lavrentyeva Pr., 10.
⁵ Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences, Kemerovo, Russian Federation, Leningradsky Pr., 10.
⁶ Kemerovo Regional Oncology Center, Kemerovo, Russian Federation, Volgogradskaya St., 35.
⁷ Institute for Medical Research and Occupational Health, Zagreb, Croatia, Ksaverska c 2.

PMID: 33454779
DOI: 10.1093/mutage/geab004

Abstract

Recent findings indicate that the microbiome may have significant impact on the development of lung cancer by its effects on inflammation, dysbiosis or genome damage. The aim of this study was to compare the sputum microbiome of lung cancer (LC) patients with the chromosomal aberration (CA) and micronuclei (MN) frequency in peripheral blood lymphocytes. In the study, the taxonomic composition of the sputum microbiome of 66 men with untreated LC were compared with 62 control subjects with respect to CA and MN frequency and centromere fluorescence in situ hybridisation analysis. Results showed a significant increase in CA (4.11 ± 2.48% versus 2.08 ± 1.18%) and MN (1.53 ± 0.67% versus 0.87 ± 0.49%) frequencies, respectively, in LC patients as compared to control subjects. The higher frequency of centromeric positive MN of LC patients was mainly due to aneuploidy. A significant increase in Streptococcus, Bacillus, Gemella and Haemophilus in LC patients was detected, in comparison to the control subjects while 18 bacterial genera were significantly reduced, which indicates a decrease in the beta diversity in the microbiome of LC patients. Although, the CA frequency in LC patients is significantly associated with an increased presence of the genera Bacteroides, Lachnoanaerobaculum, Porphyromonas, Mycoplasma and Fusobacterium in their sputum, and a decrease for the genus Granulicatella after application of false discovery rate correction, significance was not any more present. The decrease of MN frequency of LC patients is significantly associated with an increase in Megasphaera genera and Selenomonas bovis. In conclusion, a significant difference in beta diversity of microbiome between LC and control subjects and association between the sputum microbiome composition and genome damage of LC patients was detected, thus supporting previous studies suggesting an etiological connection between the airway microbiome and LC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aneuploidy
Biodiversity
Centromere / genetics
Chromosome Aberrations / statistics & numerical data
DNA Damage*
DNA, Bacterial
Dysbiosis / microbiology
Humans
Inflammation / microbiology
Lung Neoplasms / microbiology*
Lymphocytes*
Male
Microbiota*
Micronuclei, Chromosome-Defective / statistics & numerical data
Middle Aged
RNA, Ribosomal, 16S
Respiratory System / microbiology*
Sputum / microbiology

Substances

DNA, Bacterial
RNA, Ribosomal, 16S