TFIIB-related factor 2 regulates glucose-regulated protein 78 expression in acquired middle ear cholesteatoma

Biochem Biophys Res Commun. 2021 Feb 12:540:95-100. doi: 10.1016/j.bbrc.2020.12.052. Epub 2021 Jan 13.

Abstract

Acquired middle ear cholesteatoma leads to hearing loss, ear discharge, ear pain, and more serious intracranial complications. However, there is still no effective treatment other than surgery. TFIIB-related factor 2 (BRF2) acted as a redox sensor overexpressing in oxidative stress which linked endoplasmic reticulum (ER) stress, while glucose-regulated protein 78 (GRP78) was a biomarker of ER stress in cancer, atherosclerosis and inflammation. In our study, we investigated the roles of BRF2 and GRP78 in acquired middle ear cholesteatoma. Our results revealed that the expression of BRF2 was significant increased in acquired middle ear cholesteatoma, and which was positively correlated with the expression of GRP78. In addition, BRF2 and GRP78 showed colocalization in epithelium of acquired middle ear cholesteatomas and HaCaT cells. Prolongation of LPS stimulation in HaCaT cells escalated the expression of BRF2 and GRP78. To confirm the role of BRF2 and GRP78, we transfected si-BRF2 into HaCaT cells. All results indicated that BRF2 expression positively regulates the expression of GRP78 and may participate in the pathogenesis of acquire middle ear cholesteatoma.

Keywords: Acquired middle ear cholesteatoma; BRF2; GRP78.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cholesteatoma, Middle Ear / metabolism*
  • Endoplasmic Reticulum Chaperone BiP
  • Gene Knockdown Techniques
  • Heat-Shock Proteins / deficiency
  • Heat-Shock Proteins / metabolism*
  • Humans
  • Lipopolysaccharides / immunology
  • Transcription Factor TFIIIB / deficiency
  • Transcription Factor TFIIIB / metabolism*
  • Up-Regulation

Substances

  • BRF2 protein, human
  • Endoplasmic Reticulum Chaperone BiP
  • HSPA5 protein, human
  • Heat-Shock Proteins
  • Lipopolysaccharides
  • Transcription Factor TFIIIB