The construction of an improved model of acute subdural hematoma in rats

Liang Xian; Cheng Wang; Wei Wang; Liangfeng Wei; Yaying Zhang; Weiqiang Chen; Shousen Wang

doi:10.1016/j.jneumeth.2021.109075

The construction of an improved model of acute subdural hematoma in rats

J Neurosci Methods. 2021 Mar 1:351:109075. doi: 10.1016/j.jneumeth.2021.109075. Epub 2021 Jan 12.

Authors

Liang Xian¹, Cheng Wang², Wei Wang², Liangfeng Wei³, Yaying Zhang¹, Weiqiang Chen⁴, Shousen Wang⁵

Affiliations

¹ Bengbu Medical College, Bengbu 233030, PR China.
² Fuzong Clinical Medical College of Fujian Medical University, Fuzhou 350025, PR China.
³ Department of Neurosurgery, 900 Hospital of the Joint Logistics Team, Fuzhou 350025, PR China.
⁴ Department of Neurosurgery, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, PR China.
⁵ Bengbu Medical College, Bengbu 233030, PR China; Department of Neurosurgery, 900 Hospital of the Joint Logistics Team, Fuzhou 350025, PR China. Electronic address: wshsen@126.com.

PMID: 33450332
DOI: 10.1016/j.jneumeth.2021.109075

Abstract

Background: To construct a new and improved model of acute subdural hematoma in rats.

New method: 30 male adult Sprague-Dawley rats(SD rats) were selected and randomly divided into two groups. The traditional model group was based on Miller's model construction method, and the improved model group was based on improved needle, injection site and operation method. The improved model was evaluated by comparing the physiological indicators, behavioral scores, magnetic resonance performance and HE staining results of the two groups of rats.

Results: The physical signs of the rats in the two groups were similar. The survival rate of the improved group was higher than that of the traditional group. The hematoma in the improved model was thicker and concentrated in the ipsilateral side, as revealed by HE staining and MRI. The improved method has less intrusions on the cortex around the injection site and is more stable than the traditional model.

Comparison with existing method(s): The operation difficulty of the improved model is reduced and easier. The survival rate of the improved group was higher than that of the traditional group. And the improved model will have more research possibilities.

Conclusion: The improved model is based on the traditional model. Although it has some shortcomings, it can also be used in different research fields of the traditional model. The operation for the improved model is easier to perform. And the improved model has more applications in research.

Keywords: Acute subdural hematoma; Animal model; MRI; Rat; Traumatic brain injury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cerebral Cortex
Hematoma, Subdural, Acute* / diagnostic imaging
Hematoma, Subdural, Acute* / surgery
Male
Rats
Rats, Sprague-Dawley