Rotavirus reverse genetics systems: Development and application

Yuta Kanai; Takeshi Kobayashi

doi:10.1016/j.virusres.2021.198296

Rotavirus reverse genetics systems: Development and application

Virus Res. 2021 Apr 2:295:198296. doi: 10.1016/j.virusres.2021.198296. Epub 2021 Jan 10.

Authors

Yuta Kanai¹, Takeshi Kobayashi²

Affiliations

¹ Department of Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. Electronic address: y-kanai@biken.osaka-u.ac.jp.
² Department of Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. Electronic address: tkobayashi@biken.osaka-u.ac.jp.

PMID: 33440223
DOI: 10.1016/j.virusres.2021.198296

Abstract

Rotaviruses (RVs) cause acute gastroenteritis in infants and young children. Since 2006, live-attenuated vaccines have reduced the number of RV-associated deaths; however, RV is still responsible for an estimated 228,047 annual deaths worldwide. RV, a member of the family Reoviridae, has an 11-segmented double-stranded RNA genome contained within a non-enveloped, triple layered virus particle. In 2017, a long-awaited helper virus-free reverse genetics system for RV was established. Since then, numerous studies have reported the generation of recombinant RVs; these studies verify the robustness of reverse genetics systems. This review provides technical insight into current reverse genetics systems for RVs, as well as discussing basic and applied studies that have used these systems.

Keywords: Reverse genetics system; Rotavirus.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Child
Child, Preschool
Gastroenteritis*
Humans
Infant
Reoviridae* / genetics
Reverse Genetics
Rotavirus Infections*
Rotavirus Vaccines* / genetics
Rotavirus* / genetics

Substances

Rotavirus Vaccines