New possibilities for egg white lysozyme: heat-denatured lysozyme partially inactivates select foot-and-mouth disease virus strains

Sci Rep. 2021 Jan 12;11(1):526. doi: 10.1038/s41598-020-80239-8.

Abstract

Foot-and-mouth disease (FMD) is one of the most contagious diseases of cloven-hoofed animals. Disinfectants are used to inactivate FMD virus (FMDV) in Japan. Reports that heat-denatured lysozyme inactivates bacteria as well as viruses, such as norovirus and hepatitis A virus, led us to determine its effects on FMDV. We show here that heat-denatured lysozyme partially inhibited the infectivity of FMDV O/JPN/2010-1/14C but of FMDVs A/TAI/46-1/2015 and Asia1/Shamir (ISR/3/89). Further, heat-denatured lysozyme variably reduced RNA loads of FMDVs O/JPN/2010-1/14C, O/MOG/2/Ca/BU/2017, O/Taiwan/1997, Asia1/Shamir (ISR/3/89), Asia1/TUR/49/2011, SAT1/KEN/117/2009, SAT2/SAU/6/2000 and SAT3/ZIM/3/83 but could not those of O/JPN/2000, A/TAI/46-1/2015, A22/IRQ/24/64, A15/TAI/1/60 and C/PHI/7/84. These findings indicate that heat-denatured lysozyme may serve as a new disinfectant against FMDV.

MeSH terms

  • Disinfectants*
  • Egg White / chemistry*
  • Foot-and-Mouth Disease Virus / genetics*
  • Foot-and-Mouth Disease Virus / pathogenicity*
  • Foot-and-Mouth Disease Virus / physiology
  • Hot Temperature*
  • Muramidase / isolation & purification
  • Muramidase / pharmacology*
  • Protein Denaturation*
  • RNA, Viral / metabolism
  • Virus Inactivation / drug effects*

Substances

  • Disinfectants
  • RNA, Viral
  • Muramidase