Abstract
Wound dressing composed of chitosan, based crosslinked gelatin/ polyvinyl pyrrolidone, embedded silver nanoparticles were fabricated using solution casting method. The membrane was characterized by FTIR, SEM and TGA. Glutaraldehyde (0.5 %) was used for the crosslinking of membrane components and associated with 7-folds boosted mechanical performance, 28 % more hydrolytic stability, 3-folds thickness reduction and morphological roughness. Silver nanoparticles were characterized by UV-vis, XRD and TEM for an average size of 9.9 nm. The membrane with higher concentration of silver nanoparticles showed maximum antibacterial activity against human pathogenic bacteria; and the measured inhibition zones ranged from 1.5 to 3 cm. The activity of the particles ranged from severe to complete reduction in Penicillin, Erythromycin and Macrolide family's resistance genes expression such as β-Lactamase, mecA and erm. This developed membrane can serve as promising and cost-effective system against severe diabetic and burn wound infections.
Keywords:
Bacterial resistance; Chitosan; Gelatin; Polyvinyl pyrrolidone; Silver nanoparticles; Wound dressing.
Copyright © 2020 Elsevier Ltd. All rights reserved.
MeSH terms
-
Anti-Bacterial Agents / pharmacology*
-
Bacillus subtilis / drug effects
-
Bacillus subtilis / growth & development
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Bandages*
-
Chitosan / chemistry*
-
Citrullus colocynthis / chemistry*
-
Drug Resistance, Multiple, Bacterial / drug effects
-
Drug Resistance, Multiple, Bacterial / genetics
-
Erythromycin / pharmacology
-
Escherichia coli / drug effects
-
Escherichia coli / growth & development
-
Fibroblasts / cytology
-
Fibroblasts / drug effects
-
Gelatin / chemistry*
-
Gene Expression Regulation, Bacterial / drug effects
-
Humans
-
Macrolides / pharmacology
-
Metal Nanoparticles / chemistry
-
Metal Nanoparticles / ultrastructure
-
Methyltransferases / genetics
-
Methyltransferases / metabolism
-
Microbial Sensitivity Tests
-
Penicillin-Binding Proteins / genetics
-
Penicillin-Binding Proteins / metabolism
-
Penicillins / pharmacology
-
Povidone / chemistry*
-
Primary Cell Culture
-
Pseudomonas aeruginosa / drug effects
-
Pseudomonas aeruginosa / growth & development
-
Salmonella typhi / drug effects
-
Salmonella typhi / growth & development
-
Silver / chemistry
-
Silver / pharmacology*
-
Staphylococcus aureus / drug effects
-
Staphylococcus aureus / growth & development
-
beta-Lactamases / genetics
-
beta-Lactamases / metabolism
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
Macrolides
-
Penicillin-Binding Proteins
-
Penicillins
-
mecA protein, Staphylococcus aureus
-
Silver
-
Erythromycin
-
Gelatin
-
Chitosan
-
Methyltransferases
-
ErmA protein, Bacteria
-
beta-Lactamases
-
Povidone