F-Doped Micropore/Nanorod Hierarchically Patterned Coatings for Improving Antibacterial and Osteogenic Activities of Bone Implants in Bacteria-Infected Cases

Jianhong Zhou; Bo Li; Lingzhou Zhao; Lan Zhang; Yong Han

doi:10.1021/acsbiomaterials.6b00710

F-Doped Micropore/Nanorod Hierarchically Patterned Coatings for Improving Antibacterial and Osteogenic Activities of Bone Implants in Bacteria-Infected Cases

ACS Biomater Sci Eng. 2017 Jul 10;3(7):1437-1450. doi: 10.1021/acsbiomaterials.6b00710. Epub 2017 Jan 9.

Authors

Jianhong Zhou^{1

2}, Bo Li¹, Lingzhou Zhao³, Lan Zhang¹, Yong Han¹

Affiliations

¹ State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
² Institute of Physics & Optoelectronics Technology, Baoji University of Arts and Sciences, Baoji 721016, China.
³ State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.

PMID: 33429701
DOI: 10.1021/acsbiomaterials.6b00710

Abstract

Advanced titanium (Ti) based bone implants with antibacterial and osteogenic activities are extremely needed in clinics. Herein, biomimetic hierarchical micropore/nanorod patterned coatings (MNRs) on Ti were developed, in which the nanorods revealed a fixed interrod spacing of about 70 nm and consisted of fluorine (F) incorporated Ca₉Sr₁(PO₄)₆(OH)₂ (Sr₁-HA, strontium containing hydroxyapatite) with the fixed Sr but different F content. The incorporation of F was determined by XRD, FTTR, and TEM, revealing the substitution of OH^- in Sr₁-HA to F^-. The topography, surface roughness, and hydrophilic nature of MNRs were not apparently affected by the incorporation of F. The antibacterial and osteogenic activities of MNRs were assessed in vitro and in a bacterial-infected rabbit model. Both the in vitro and in vivo results showed that F-free and ∼1 wt % F doped MNR significantly promoted osteogenic activity compared to that of Ti but showed a lack of antibacterial activity. The incorporation of a higher dose of F led to the significantly improved antibacterial and osteogenic activities, and the effects were related to the incorporated F dose. In total, compared to F-free, ∼1, ∼2, and ∼7 wt % F doped MNRs, ∼5 wt % F doped MNR significantly enhanced antibacterial and osteogenic activities, especially new bone formation and osseointegration in the bacteria-infected case, showing a high potential as the next generation of advanced bone implant.

Keywords: Sr-HA nanorod; antibacterial ability; biomimetic hierarchical topography; fluorine incorporation; mesenchymal stem cell; osseointegration.