Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

Qiaoning Wang; Hongdan Wang; Yaru Jiang; Min Lv; Xiaoyan Wang; Lingxin Chen

doi:10.1016/j.jhazmat.2020.125023

Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

J Hazard Mater. 2021 Jun 5:411:125023. doi: 10.1016/j.jhazmat.2020.125023. Epub 2021 Jan 2.

Authors

Qiaoning Wang¹, Hongdan Wang², Yaru Jiang³, Min Lv², Xiaoyan Wang⁴, Lingxin Chen⁵

Affiliations

¹ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Provincial Key Laboratory of Coastal Environmental Processes, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Provincial Key Laboratory of Coastal Environmental Processes, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
³ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Provincial Key Laboratory of Coastal Environmental Processes, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
⁴ School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
⁵ CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Provincial Key Laboratory of Coastal Environmental Processes, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: lxchen@yic.ac.cn.

PMID: 33429311
DOI: 10.1016/j.jhazmat.2020.125023

Abstract

Sulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1-10 μg/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 μg/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2-2 could be activated under SMX stress of 10 μg/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics.

Keywords: Biotransformation; Resistance; Sulfamethoxazole; Transcriptome; Vibrio diabolicus strain L2–2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents
Biotransformation
Sulfamethoxazole*
Vibrio* / genetics

Substances

Anti-Bacterial Agents
Sulfamethoxazole

Supplementary concepts

Vibrio diabolicus