miR-429-3p/ LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway

Xiaohuan Chao; Lijin Guo; Qi Wang; Weiling Huang; Manqing Liu; Kang Luan; Jinqi Jiang; Shudai Lin; Qinghua Nie; Wen Luo; Xiquan Zhang; Qingbin Luo

doi:10.3389/fcell.2020.595637

miR-429-3p/ LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway

Front Cell Dev Biol. 2020 Dec 21:8:595637. doi: 10.3389/fcell.2020.595637. eCollection 2020.

Authors

Xiaohuan Chao^{1

2

3}, Lijin Guo^{1

2

3}, Qi Wang^{1

2

3}, Weiling Huang^{1

2

3}, Manqing Liu^{1

2

3}, Kang Luan^{1

2

3}, Jinqi Jiang^{1

2

3}, Shudai Lin^{1

2

3}, Qinghua Nie^{1

2

3}, Wen Luo^{1

2

3}, Xiquan Zhang^{1

2

3}, Qingbin Luo^{1

2

3}

Affiliations

¹ Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.
² Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.
³ College of Animal Science, South China Agricultural University, Guangzhou, China.

Abstract

To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens (n = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas LPIN1 expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas LPIN1 exerted an opposite effect. Notably, we found that the miR-429-3p/LPIN1 axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/LPIN1 axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.

Keywords: PPARγ pathway abstract; RNA-Seq; miR-429-3p/LPIN1 axis; preadipocyte differentiation; preadipocyte proliferation.