Proteomic Changes in Antioxidant System in Strawberry During Ripening

Jun Song; Leslie CampbellPalmer; Mindy Vinqvist-Tymchuk; Sherry Fillmore; Charles Forney; Honghui Luo; Zhaoqi Zhang

doi:10.3389/fpls.2020.594156

Proteomic Changes in Antioxidant System in Strawberry During Ripening

Front Plant Sci. 2020 Dec 23:11:594156. doi: 10.3389/fpls.2020.594156. eCollection 2020.

Authors

Jun Song¹, Leslie CampbellPalmer¹, Mindy Vinqvist-Tymchuk¹, Sherry Fillmore¹, Charles Forney¹, Honghui Luo², Zhaoqi Zhang²

Affiliations

¹ Agriculture and Agri-Food Canada, Kentville Research and Development Centre, Kentville, NS, Canada.
² College of Horticulture, South China Agriculture University, Guangzhou, China.

Abstract

To investigate the strawberry antioxidant defense system during fruit ripening, a targeted quantitative proteomic approach using multiple reaction monitoring (MRM) was developed to investigate targeted proteins in the antioxidant enzyme system in strawberry fruit. We investigated 46 proteins and isoforms with 73 identified peptides which may be involved in this antioxidant enzyme system. Among the proteins that changed during ripening, aldo/keto reductase (AKR), superoxide dismutase (SOD) and glutathione transferase (GT) increased significantly, while dehydroascorbate reductase, 2-Cys peroxiredoxin, catalase (CAT), 1-Cys peroxiredoxin and L-ascorbate peroxidase (APX) decreased significantly. These results suggest that fruit ripening of strawberry activates the enzymes of an SOD/glutathione metabolism system. The methodologies used in this study will be useful for systematically characterizing the role of antioxidant enzymes in fruit ripening of other plants.

Keywords: Fragaria × ananassa; OFFGEL; fruit ripening; multiple reaction monitoring; proteomics; quality.