Background: The aim of this work was to study the possible synergic effect between mixtures with iron leonardite humate (L/Fe3+ ) and synthetic chelates iron (Ch/Fe3+ : o,oEDDHA /Fe3+ or HBED/Fe3+ ), and to reevaluate the classical chelate shuttle-effect model. Different molar ratios of L/Fe3+ :Ch/Fe3+ , different doses, and different sampling times were used in hydroponic and soil experiments using soybean (Glycine max) as a model Strategy I crop in calcareous conditions. Ligand competition between the humate and chelating agents was also examined.
Results: Iron humate participates in the chelate shuttle mechanism, providing available Fe to the chelating agent and then to the plants, showing a slight synergic effect. After a few days, the contribution of the chelates to the Fe nutrition decreases substantially, but the contribution of the humates is maintained.
Conclusions: The most efficient ratio was two parts of iron humates and one part of iron chelate. In particular, HBED/Fe3+ was the most suitable iron chelate because its lasting effect fits the iron humate long-term effect better. The soluble iron in soil increased and the shoot-to-root iron translocation improved due to a synergic effect by a shuttle effect exerted by iron chelate in the mixture. © 2021 Society of Chemical Industry.
Keywords: Leonardite; iron humate; iron isotopes; iron nutrition; shuttle effect; soybean.
© 2021 Society of Chemical Industry.