Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level

Emmanuelle Sachon; Astrid Walrant; Sandrine Sagan; Sophie Cribier; Nicolas Rodriguez

doi:10.1016/j.abb.2021.108751

Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level

Arch Biochem Biophys. 2021 Mar 15:699:108751. doi: 10.1016/j.abb.2021.108751. Epub 2021 Jan 7.

Authors

Emmanuelle Sachon¹, Astrid Walrant¹, Sandrine Sagan¹, Sophie Cribier², Nicolas Rodriguez¹

Affiliations

¹ Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005, Paris, France.
² Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005, Paris, France. Electronic address: sophie.cribier@sorbonne-universite.fr.

PMID: 33421380
DOI: 10.1016/j.abb.2021.108751

Abstract

Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques and membrane models. This review presents some of the most recent original and innovative developments in this field, namely droplet interface bilayers (DIBs), new fluorescence approaches, force measurements, and photolabelling.

Keywords: Antimicrobial peptides; Biomembrane force probe; Cell penetrating peptides; Droplet interface bilayers; Fluorescence; Photolabelling.

Publication types

Review

MeSH terms

Amino Acid Sequence
Antimicrobial Cationic Peptides / chemistry
Antimicrobial Cationic Peptides / metabolism*
Cell Membrane / chemistry
Cell Membrane / metabolism*
Cell-Penetrating Peptides / chemistry
Cell-Penetrating Peptides / metabolism*
Fluorescent Dyes / chemistry
Lipid Bilayers / chemistry
Lipid Bilayers / metabolism
Microscopy, Fluorescence / methods
Photoaffinity Labels / chemistry
Spectrometry, Fluorescence / methods

Substances

Antimicrobial Cationic Peptides
Cell-Penetrating Peptides
Fluorescent Dyes
Lipid Bilayers
Photoaffinity Labels