[Safety of eight nucleic acid dyeson]

Wen Yu; Lin An; Yiwen Chen; Xiu Ren; Haipeng Luo; Na Liu; Shenghui Cui; Jingyun Li

doi:10.19813/j.cnki.weishengyanjiu.2020.06.011

[Safety of eight nucleic acid dyeson]

Wei Sheng Yan Jiu. 2020 Nov;49(6):938-948. doi: 10.19813/j.cnki.weishengyanjiu.2020.06.011.

[Article in Chinese]

Authors

Wen Yu¹, Lin An¹, Yiwen Chen¹, Xiu Ren¹, Haipeng Luo¹, Na Liu¹, Shenghui Cui¹, Jingyun Li¹

Affiliation

¹ National Institutes for Food and Drug Control, Beijing 100050, China.

PMID: 33413769
DOI: 10.19813/j.cnki.weishengyanjiu.2020.06.011

Abstract

Objective: To study the safety of eight nucleic acid dyes: EB, GelGreen, GoldView, GeneGreen, SYBRGreen I, GelRed, SYBRSafe and SYBRGold.

Methods: Salmonell atyphimurium TA97, TA98, TA100 and TA102 involved in the minimum inhibitory concentration(MIC) of the 8 nucleic acid dyes by the Broth dilution, which follows Clinical and Laboratory Standards Institute. The experiment tests the reverse mutation of bacteria with the 8 nucleic acid dyes, which follows GB 15193. 4-2014.

Results: EB and its metabolites show significant mutagenicity on TA97, TA98, TA100 and TA102. SYBRGold and its metabolites do not show mutagenicity on TA97, TA98, TA100 and TA102. The other six dyes show varying mutagenicity on TA97, TA98 and TA102, meanwhile show no mutagenicity on TA100.

Conclusion: This research shows that, except EB, the other dyes show no mutagenicity under working concentration, but show varying mutagenicity under high concentration. SYBRSafe and SYBRGreenI are highly toxic, and the operators must have high precautions when making diluted solution of them.

Keywords: food safety; mutagenicity; nucleic acid dyes; safety.

MeSH terms

Mutagenicity Tests
Mutagens / toxicity
Mutation
Nucleic Acids*
Salmonella typhimurium* / genetics

Substances

Mutagens
Nucleic Acids