AOTF-based hyperspectral imaging phase microscopy

Konstantin B Yushkov; Justine Champagne; Jean-Claude Kastelik; Oleg Yu Makarov; Vladimir Ya Molchanov

doi:10.1364/BOE.406155

AOTF-based hyperspectral imaging phase microscopy

Biomed Opt Express. 2020 Nov 10;11(12):7053-7061. doi: 10.1364/BOE.406155. eCollection 2020 Dec 1.

Authors

Konstantin B Yushkov¹, Justine Champagne², Jean-Claude Kastelik², Oleg Yu Makarov¹, Vladimir Ya Molchanov¹

Affiliations

¹ National University of Science and Technology "MISIS", 4 Leninsky prospekt, Moscow, 119049, Russia.
² IEMN UMR 8520, Université Polytechnique des Hauts-de-France, Campus Mont Houy, Valenciennes, 59313, France.

Abstract

Phase imaging microscopy with incoherent object illumination is convenient and affordable for biomedical research and clinics since it provides easy integration with a variety of bright-field optical microscopes. We report the design of a new hyperspectral imaging system based on a combination of a spatial light modulator (SLM) and an acousto-optic tunable filter (AOTF) for phase imaging microscopy. Contrast of phase-only objects originates from matched spectral and spatial filtering performed by the SLM and the AOTF located in Fourier-conjugate optical planes in the back-end of the optical system. The system is designed as an add-on to a standard optical microscope with incoherent diascopic sample illumination.