Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Weijian Zong; Runlong Wu; Shiyuan Chen; Junjie Wu; Hanbin Wang; Zhe Zhao; Guoqing Chen; Rui Tu; Danlei Wu; Yanhui Hu; Yangyang Xu; Yao Wang; Zhuoli Duan; Haitao Wu; Yunfeng Zhang; Jue Zhang; Aimin Wang; Liangyi Chen; Heping Cheng

doi:10.1038/s41592-020-01024-z

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Nat Methods. 2021 Jan;18(1):46-49. doi: 10.1038/s41592-020-01024-z. Epub 2021 Jan 6.

Authors

Weijian Zong^#^{1

2

3}, Runlong Wu^#⁴, Shiyuan Chen¹, Junjie Wu⁵, Hanbin Wang⁶, Zhe Zhao⁷, Guoqing Chen¹, Rui Tu¹, Danlei Wu⁸, Yanhui Hu⁸, Yangyang Xu⁸, Yao Wang⁶, Zhuoli Duan⁹, Haitao Wu⁷, Yunfeng Zhang¹⁰, Jue Zhang^{5

6}, Aimin Wang^{11

12}, Liangyi Chen^{13

14

15}, Heping Cheng^{1

16}

Affiliations

¹ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
² Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing, China.
³ Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway.
⁴ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. rlwu@pku.edu.cn.
⁵ College of Engineering, Peking University, Beijing, China.
⁶ Academy of Advanced Interdisciplinary Study, Peking University, Beijing, China.
⁷ Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, China.
⁸ Beijing Transcend Vivoscope Biotech Co., Ltd, Beijing, China.
⁹ Department of Neurology, Southern Medical University, Guangzhou, China.
¹⁰ Department of Electronics, Peking University, Beijing, China.
¹¹ Department of Electronics, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹² State Key Laboratory of Advanced Optical Communication System and Networks, Peking University, Beijing, China. wangaimin@pku.edu.cn.
¹³ State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China. lychen@pku.edu.cn.
¹⁴ PKU-IDG/McGovern Institute for Brain Research, Beijing, China. lychen@pku.edu.cn.
¹⁵ Beijing Academy of Artificial Intelligence, Beijing, China. lychen@pku.edu.cn.
¹⁶ Research Unit of Mitochondria in Brain Diseases, Chinese Academy of Medical Sciences, PKU-Nanjing Institute of Translational Medicine, Nanjing, China.

^# Contributed equally.

PMID: 33408404
DOI: 10.1038/s41592-020-01024-z

Abstract

We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm³ at a lateral resolution of ~1 μm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal
Brain / cytology
Brain / diagnostic imaging*
Brain / physiology*
Cytological Techniques
Locomotion
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton / methods*
Miniaturization / methods*
Neuroimaging / methods*