Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Thi Bich Thuy Do; Bao Khanh Tran; Thi Van Thi Tran; Trung Hieu Le; Margo Cnockaert; Peter Vandamme; Thi Hong Chuong Nguyen; Chinh Chien Nguyen; Sung Hyun Hong; Soo Young Kim; Quyet Van Le

doi:10.1021/acsomega.0c05256

Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

ACS Omega. 2020 Dec 17;5(51):33387-33394. doi: 10.1021/acsomega.0c05256. eCollection 2020 Dec 29.

Authors

Affiliations

¹ Faculty of Engineering and Food Technology, Hue University of Agriculture and Forestry, Hue University, Thua Thien Hue 530000, Vietnam.
² Hue University of Sciences, Hue University, Thua Thien Hue 530000, Vietnam.
³ Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
⁴ Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam.
⁵ Faculty of Environmental and Chemical Engineering, Duy Tan University, Da Nang 550000, Vietnam.
⁶ Department of Materials Science and Engineering, Institute of Green Manufacturing Technology, Korea University, 145, Anam-ro Seongbuk-gu, Seoul 02841, Republic of Korea.

Abstract

This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated Lactobacillus plantarum W1 (EPS-W1), which belongs to genus Lactobacillus, is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopolysaccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of →6)-d-Glcp-(1→; →3)-d-Manp-(1→; →3)-d-Glcp-(1→ and a branch of →6)-d-Manp-(1→; →2)-d-Glcp-(1→. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications.