Decompression Process of Glycerol Shock Treatment Can Overcome Endo-Lysosomal Barriers for Intracellular Delivery

Shupeng Wang; Shaohua Jin; Guangzhi Li; Rui Sun; Qinghai Shu; Song Wu

doi:10.1021/acsomega.0c04771

Decompression Process of Glycerol Shock Treatment Can Overcome Endo-Lysosomal Barriers for Intracellular Delivery

ACS Omega. 2020 Dec 16;5(51):33133-33139. doi: 10.1021/acsomega.0c04771. eCollection 2020 Dec 29.

Authors

Shupeng Wang^{1

2}, Shaohua Jin¹, Guangzhi Li², Rui Sun², Qinghai Shu¹, Song Wu²

Affiliations

¹ School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.
² The Affiliated Luohu Hospital of Shenzhen University, Shenzhen University, Shenzhen 518001, China.

Abstract

The glycerol shock treatment has been used to improve the calcium phosphate transfection efficacy for several decades because of its high effectiveness and low toxicity. However, the mechanism of glycerol shock treatment is still obscure. In this study, the endo-lysosomal leakage assay demonstrated that the decompression process of glycerol shock treatment could enhance endo-lysosomal membrane permeabilization, which resulted in facilitating endo-lysosomal escape for effective intracellular delivery. The enhanced decompression treatment derived from glycerol shock treatment could increase the change of osmotic pressure further, which showed higher efficacy for intracellular delivery. Herein, we speculated that the endo-lysosomal swelling originated from the decompression process of glycerol shock treatment could cause endo-lysosomal damage.