Biosensing Detection of the SARS-CoV-2 D614G Mutation

Yang Zhang; Hui Xi; Mario Juhas

doi:10.1016/j.tig.2020.12.004

Biosensing Detection of the SARS-CoV-2 D614G Mutation

Trends Genet. 2021 Apr;37(4):299-302. doi: 10.1016/j.tig.2020.12.004. Epub 2020 Dec 24.

Authors

Yang Zhang¹, Hui Xi², Mario Juhas³

Affiliations

¹ College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, China. Electronic address: zhangyang07@hit.edu.cn.
² College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, China.
³ Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.

Abstract

The emergence of a mutant strain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with an amino acid change from aspartate to a glycine residue at position 614 (D614G) has been reported and this mutant appears to be now dominant in the pandemic. Efficient detection of the SARS-CoV-2 D614G mutant by biosensing technologies is therefore crucial for the control of the pandemic.

Keywords: CRISPR-Cas; hybridization; loop-mediated isothermal amplification (LAMP); nanopore; restriction endonucleases; spike.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Biosensing Techniques*
Mutation*
SARS-CoV-2 / genetics*
Spike Glycoprotein, Coronavirus / genetics*

Substances

Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2