Draft Genome Sequence of Enterobacter sp. AS-1, a Potential Eurytrophic Recombination Host

Yuki Iwasaki; Yuya Itoiri; Sota Ihara; Hironaga Akita; Mamoru Oshiki; Zen-Ichiro Kimura

doi:10.7150/jgen.53040

Draft Genome Sequence of Enterobacter sp. AS-1, a Potential Eurytrophic Recombination Host

J Genomics. 2021 Jan 1:9:6-9. doi: 10.7150/jgen.53040. eCollection 2021.

Authors

Yuki Iwasaki¹, Yuya Itoiri¹, Sota Ihara¹, Hironaga Akita², Mamoru Oshiki³, Zen-Ichiro Kimura¹

Affiliations

¹ National Institute of Technology (KOSEN), Kure College (NIT, Kure), 2-2-11, Agaminami, Kure, Hiroshima 737-8506, Japan.
² Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32, Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.
³ Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13, West 8, Sapporo, Hokkaido, 060-8628, Japan.

Abstract

Strain AS-1 was isolated from laboratory-scale activated sludge collected in Japan. This strain not only grows on rich medium, including R2A medium, but also forms colonies on medium lacking organic matter other than agar (water agar), indicating it could be used as a eurytrophic recombinant host in material production processes. Here, we present a draft genome sequence of Enterobacter sp. AS-1, which consists of a total of 24 contigs containing 5,207,146 bp, with a GC content of 55.64%, and comprising 4,921 predicted coding sequences. Based on 16S rRNA gene sequence analysis, strain AS-1 was designated as Enterobacter sp. AS-1.

Keywords: Enterobacter; Eurytrophic; Recombination host; Whole genome sequence.