Effect of the AM Fungus Sieverdingia tortuosa on Common Vetch Responses to an Anthracnose Pathogen

Tingting Ding; Weizhen Zhang; Yingde Li; Tingyu Duan

doi:10.3389/fmicb.2020.542623

Effect of the AM Fungus Sieverdingia tortuosa on Common Vetch Responses to an Anthracnose Pathogen

Front Microbiol. 2020 Dec 18:11:542623. doi: 10.3389/fmicb.2020.542623. eCollection 2020.

Authors

Tingting Ding^{1

2

3}, Weizhen Zhang^{1

2

3}, Yingde Li^{1

2

3}, Tingyu Duan^{1

2

3}

Affiliations

¹ Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou, China.
² College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
³ Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou, China.

Abstract

Colletotrichum lentis Damm causes anthracnose in Vicia sativa L, otherwise known as common vetch. It was first reported in China in 2019. This study evaluates the effects of the arbuscular mycorrhizal (AM) fungus Sieverdingia tortuosa (N.C. Schenck & G.S. Sm.) Błaszk., Niezgoda, & B.T. Goto on growth and disease severity in common vetch. Our main finding is that the AM fungus increased root biomass and reduced anthracnose severity of common vetch. Responses correlated with defense, such as chitinase activity, polyphenol oxidase (PPO) activity, the concentrations of jasmonic acid and proline, and the expression of resistance-related genes (e.g., upregulated "signal transduction," "MAPK signaling pathway," "chitinase activity," "response to stress," and the KEGG pathways "phenylpropanoid biosynthesis," "MAPK signaling pathways," and "plant-pathogen interactions"), were also affected These findings provide insight into the mechanism by which this AM fungus regulates the defense response of common vetch to C. lentis.

Keywords: Colletotrichum lentis; RNA-seq; Sieverdingia tortuosa; common vetch; disease.