Abstract
Prevotella nigrescens is an oral pathogen that is frequently observed in the subgingival plaque of periodontitis patients. Interleukin-1β (IL-1β) is known to be involved in the immunopathology of periodontal diseases and has been implicated in the destruction of bone. In this study, we investigated the mechanism of IL-1β production by P. nigrescens in murine bone marrow-derived dendritic cells (BMDCs). Our results showed that a host receptor, Toll-like receptor 2 (TLR2), but not TLR4 is required for pro-IL-1β induction and nucleotide-binding oligomerization domain like receptor pyrin domain containing 3 (NLRP3) priming in BMDCs in response to P. nigrescens and activation of the NLRP3 inflammasome is necessary for processing of pro-IL-1β into mature IL-1β. In addition, an inhibitor assay revealed that production of reactive oxygen species, P2X7R activity, and release of cathepsin B are involved in IL-1β production in BMDCs in response to P. nigrescens.
Keywords:
BMDCs; IL-1β; NLRP3 inflammasome; Prevotella nigrescens; TLR2.
© The author(s).
MeSH terms
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Animals
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Cathepsin B / metabolism
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Cells, Cultured
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Disease Models, Animal
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Gram-Negative Bacterial Infections / immunology*
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Gram-Negative Bacterial Infections / microbiology
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Humans
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Inflammasomes / immunology
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Inflammasomes / metabolism
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Interleukin-1beta / metabolism*
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Mice
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NLR Family, Pyrin Domain-Containing 3 Protein / genetics
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NLR Family, Pyrin Domain-Containing 3 Protein / metabolism*
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Periodontitis / immunology*
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Periodontitis / microbiology
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Prevotella nigrescens / immunology*
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Primary Cell Culture
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Reactive Oxygen Species / metabolism
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Receptors, Purinergic P2X7 / metabolism
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Signal Transduction / genetics
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Signal Transduction / immunology
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Toll-Like Receptor 2 / genetics
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Toll-Like Receptor 2 / metabolism*
Substances
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IL1B protein, mouse
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Inflammasomes
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Interleukin-1beta
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NLR Family, Pyrin Domain-Containing 3 Protein
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Nlrp3 protein, mouse
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P2rx7 protein, mouse
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Reactive Oxygen Species
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Receptors, Purinergic P2X7
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Tlr2 protein, mouse
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Toll-Like Receptor 2
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Cathepsin B
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Ctsb protein, mouse