Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing

S Merkert; M-C Jaboreck; L Engels; M N H Malik; G Göhring; F Pessler; U Martin; R Olmer

doi:10.1016/j.scr.2020.102135

Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing

Stem Cell Res. 2020 Dec 22:50:102135. doi: 10.1016/j.scr.2020.102135. Online ahead of print.

Authors

S Merkert¹, M-C Jaboreck², L Engels², M N H Malik³, G Göhring⁴, F Pessler³, U Martin², R Olmer²

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany. Electronic address: merkert.sylvia@mh-hannover.de.
² Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany.
³ TWINCORE Centre for Experimental and Clinical Infection Research, Centre for Individualized Infection Medicine, 30625 Hannover, Germany; Helmholtz-Centre for Infection Research Braunschweig, 38124 Braunschweig, Germany.
⁴ Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.

PMID: 33383405
DOI: 10.1016/j.scr.2020.102135

Abstract

Interferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of IFN-I signalling as well as enhanced antiviral protection. Here, we have generated ISG15 knockout clones from human iPSCs, which provide useful cell resources to study mechanisms of ISG15 deficiency and gain more insight into the biological function of ISG15.