A disc-chip based high-throughput acute toxicity detection system

Xinlian Zhang; Xi Chen; Yuhan Yao; Xiaona Shang; Huijun Lu; Wang Zhao; Sixiu Liu; Jianmin Chen; Guodong Sui

doi:10.1016/j.talanta.2020.121867

A disc-chip based high-throughput acute toxicity detection system

Talanta. 2021 Mar 1:224:121867. doi: 10.1016/j.talanta.2020.121867. Epub 2020 Nov 5.

Authors

Xinlian Zhang¹, Xi Chen¹, Yuhan Yao¹, Xiaona Shang¹, Huijun Lu¹, Wang Zhao¹, Sixiu Liu¹, Jianmin Chen¹, Guodong Sui²

Affiliations

¹ Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science & Engineering, Fudan University, 2205 Songhu Road, Shanghai, 200433, China.
² Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science & Engineering, Fudan University, 2205 Songhu Road, Shanghai, 200433, China; Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CICAEET), Nanjing University of Information Science & Technology, Nanjing, 210044, China. Electronic address: gsui@fudan.edu.cn.

PMID: 33379077
DOI: 10.1016/j.talanta.2020.121867

Abstract

Acute toxicity assay presents vital significance in modern environmental monitoring, including online detection and in-situ assay for emergency events. Although photobacteria related detection methods were established and verified in the past decades with combination of photomultiplier tube (PMT), the price and size of PMT sensor hampered application of rapid acute toxicity assay and detection system miniaturization, especially in the resource-limited occasions. Wide application of smartphones with great low-light performance cameras could be used in photobacteria-based toxicity assay instead of the PMT methods. Herein a box-type portable detection system had been successfully established, including a disc-chip for detection, detection device, and smartphones with a high-performance camera. The system performed well showing stable temperature and rotation control. Results captured by CMOS-based camera presented a linear relationship with PMT-based detection method. An image progress algorithm was also established and tested by series diluted zinc sulfate solution as a reference substance. The system also performed well for toxicity analysis for real Atmospheric particle matter sample. The system could be used in some environmental monitoring scenarios as an alternative solution.

Keywords: Acute toxicity; CMOS; Disc-chip; Microfluidics; Photobacteria; Smartphone.

MeSH terms

Miniaturization
Smartphone*