Preparation and application of peptide molecularly imprinted material based on mesoporous metal-organic framework

Abstract

In this study, a new molecularly imprinted material, MIP@UiO-66-NH₂, was synthesized with glutathione (GSH) as template and mesoporous metal organic framework (UiO-66-NH₂) as matrix. The molecularly imprinted polymer was modified on the surface and into the pores of the UiO-66-NH₂ by surface molecular imprinting method with thin polymer layer. Based on high specific surface area (1091.93 m² g^-1) and appropriate pore size (35 nm) of the ordered mesoporous UiO-66-NH₂, the adsorption capacity for GSH reached 94.43 mg g^-1, and the adsorption equilibrium could be achieved within 30 min. The adsorption isotherm data of MIP@UiO-66-NH₂ could be described well by Freundlich model and the kinetic data complied well with pseudo-second-order model. In addition, the MIP@UiO-66-NH₂ showed low adsorption capacity to GSH structural analogs (Q_L-cys = 6.51 mg g^-1), suggesting great selectivity for GSH recognition. Finally, the MIP@UiO-66-NH₂ was successfully applied for selective separation of GSH from BSA, skim milk and egg white tryptic digest.

Keywords: Adsorption; Metal-organic framework; Molecularly imprinted material; Peptides.