Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy

STAR Protoc. 2020 Dec 16;1(3):100215. doi: 10.1016/j.xpro.2020.100215. eCollection 2020 Dec 18.

Abstract

Mosaic analysis with double markers (MADM) technology enables concomitant fluorescent cell labeling and induction of uniparental chromosome disomy (UPD) with single-cell resolution. In UPD, imprinted genes are either overexpressed 2-fold or are not expressed. Here, the MADM platform is utilized to probe imprinting phenotypes at the transcriptional level. This protocol highlights major steps for the generation and isolation of projection neurons and astrocytes with MADM-induced UPD from mouse cerebral cortex for downstream single-cell and low-input sample RNA-sequencing experiments. For complete details on the use and execution of this protocol, please refer to Laukoter et al. (2020b).

Keywords: Flow Cytometry/Mass Cytometry; Genetics; Neuroscience; Single Cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes
  • Biomarkers
  • Brain / cytology*
  • Cell Separation / methods
  • Chromosomes
  • Exome Sequencing
  • Flow Cytometry / methods
  • Fluorescent Antibody Technique / methods*
  • Genomic Imprinting
  • Mice
  • Mosaicism
  • Phenotype
  • Single-Cell Analysis / methods
  • Software
  • Uniparental Disomy / cytology*

Substances

  • Biomarkers