Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Kei Takahashi; Shimpei I Kubota; Shogo Ehata; Hiroki R Ueda; Kohei Miyazono

doi:10.1016/j.xpro.2020.100191

Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

STAR Protoc. 2020 Nov 26;1(3):100191. doi: 10.1016/j.xpro.2020.100191. eCollection 2020 Dec 18.

Authors

Kei Takahashi¹, Shimpei I Kubota¹, Shogo Ehata¹, Hiroki R Ueda^{2

3}, Kohei Miyazono¹

Affiliations

¹ Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
² Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
³ Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, 1-3, Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).

Keywords: Cancer; Microscopy; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Imaging, Three-Dimensional / methods
Mice
Neoplasms / diagnostic imaging*
Optical Imaging / methods
Specimen Handling / methods*