Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells

Veronika Sander; Aneta Przepiorski; Amanda E Crunk; Neil A Hukriede; Teresa M Holm; Alan J Davidson

doi:10.1016/j.xpro.2020.100150

Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells

STAR Protoc. 2020 Oct 29;1(3):100150. doi: 10.1016/j.xpro.2020.100150. eCollection 2020 Dec 18.

Authors

Veronika Sander¹, Aneta Przepiorski², Amanda E Crunk², Neil A Hukriede², Teresa M Holm¹, Alan J Davidson¹

Affiliations

¹ Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand.
² Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Abstract

Kidney organoids represent a physiologically advanced model for studying the mechanisms of kidney development and disease. Here, we describe a simple two-step protocol for the differentiation of human pluripotent stem cells into kidney organoids. Our approach involves suspension culture that allows for rapid and cost-effective bulk production of organoids, which is well suited for large-scale assays such as drug screening. The organoids correspond to fetal human kidney tissue and may be of limited use for modeling adult kidney function. For complete details on the use and execution of this protocol, please refer to Przepiorski et al. (2018).

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Culture Techniques / methods*
Cells, Cultured
Embryoid Bodies / cytology
Humans
Kidney / cytology*
Organoids / cytology*
Paraffin Embedding
Pluripotent Stem Cells / cytology*

Abstract

Publication types

MeSH terms

Grants and funding