The complete mitochondrial genome of sugarcane (Saccharum spp.) variety FN15

Xiaolan Liu; Ze Yin; Ying Liu; Zhu Li; Mingli Yan; Youxiong Que; Liping Xu; Dinggang Zhou

doi:10.1080/23802359.2020.1768926

The complete mitochondrial genome of sugarcane (Saccharum spp.) variety FN15

Mitochondrial DNA B Resour. 2020 May 27;5(3):2163-2165. doi: 10.1080/23802359.2020.1768926. eCollection 2020.

Authors

Xiaolan Liu¹, Ze Yin¹, Ying Liu¹, Zhu Li², Mingli Yan^{1

3}, Youxiong Que², Liping Xu², Dinggang Zhou^{1

2

3}

Affiliations

¹ Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan, China.
² Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China.
³ Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-polluted Soils, College of Hunan Province, Xiangtan, China.

Abstract

The complete mitogenome of Saccharum spp. hybrid FN15 was successfully sequenced. It contains two distinct circular chromosomes, Chromosome 1 and Chromosome 2. The former is 301,533 bp in length with the GC content of 43.90%, and 7.12% of genome (21,468 nucleotides) are coding DNA while 92.88% of genome (280,065 nucleotides) are intergenic region. The latter is 144,744 bp in length with the GC content of 43.57%, and 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% of genome (132,879 nucleotides) are intergenic region. Besides, Chromosome 1 contains 22 protein-coding genes (four atp genes, three ccm genes, three cox genes, one mat gene, one mtt gene, six nad genes and four rps genes), and 21 non-coding genes (15 tRNA and six rRNAs), whereas in Chromosome 2, there are 13 protein-coding genes (two atp genes, one ccm gene, one cob gene, one cox gene, one rpl gene, four nad genes and three rps genes) and five tRNA genes. Maximum Likelihood phylogenetic analysis demonstrated that FN15 is close with S. spp. hybrid ROC22, S. officinarum Khon Kaen 3 and S. bicolor species. This complete mitochondrial genome will provide essential DNA molecular data for further phylogenetic and evolutionary analysis for Saccharum.

Keywords: Saccharum spp; mitogenome; phylogenetic analysis; sugarcane.

Grants and funding

This work was supported by National Natural Science Foundation of China [Grant No. 31701491], the Open Funding of National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University [Grant No. 2017.6.2], the Natural Science Foundation of Hunan Province, China [Grant No. 2019JJ50176], the Scientific Research Funding of Education Department of Hunan Province, China [Grant No. 17C0653] and Scientific Research Funding of Hunan University of Science and Technology [Grant No. E51761].