The mitochondrial genome analysis of Paragnetina indentata (Plecoptera: Perlidae)

Mitochondrial DNA B Resour. 2019 Dec 9;5(1):44-45. doi: 10.1080/23802359.2019.1693927.

Abstract

In this study, the entire mitochondrial genome of Paragnetina indentata was sequenced. The circular mitochondrial genome was the first mitochondrial genome representing the genus Paragnetina, which consists of 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region with the length of 15,885 bp. In the complete sequence, the A + T content for 64.1% (A for 33.6%, T for 30.5%, C for 23.3%, and G for 12.7%). Among the 13 PCGs, 11 start codons is ATN and the start codon of ND1, ND2, and ND5 genes is TTG, GTG, and GTG, respectively. In addition, 11 of the PCGs used conservative termination codon TAA or TAG, except for COII and ND5 which terminated by the single T. By using the Bayesian inference (BI) and maximum-likelihood (ML) methods, the phylogenetic relationship showed that P. indentata was closely related to Togoperla sp. and the species of Perlinae were clustered in a clade.

Keywords: Mitochondrial genome; Paragnetina indentata; phylogenetics.

Grants and funding

This work was supported by the National Natural Science Foundation of China [No. 31970402], the Innovation and Entrepreneurship Training Program for College Students in Henan Province [No. S201910467003], and the Innovation and Entrepreneurship Training Program in Henan Institute of Science and Technology [No. 2019CX088].