Chloroplast genome structure and phylogenetic position of Ruppia sinensis Shuo Yu & den Hartog

Shuo Yu; Miao-Miao Shi; Kai Jiang

doi:10.1080/23802359.2019.1662746

Chloroplast genome structure and phylogenetic position of Ruppia sinensis Shuo Yu & den Hartog

Mitochondrial DNA B Resour. 2019 Sep 5;4(2):2916-2917. doi: 10.1080/23802359.2019.1662746.

Authors

Shuo Yu¹, Miao-Miao Shi², Kai Jiang^{3

4}

Affiliations

¹ Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai, China.
² Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
³ Shanghai Chenshan Botanical Garden, Shanghai, China.
⁴ School of Ecological and Environmental Sciences, Shanghai Key Lab of Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai, China.

Abstract

Ruppia is widely distributed in marine and inland saline habitats in temperate and tropical regions. In this study, the complete chloroplast genome sequence of R. sinensis was successfully obtained using Illumina sequencing. The full length of the chloroplast genome length was 158,897 bp with a typical quadripartite structure: one large single copy (LSC) region (88,952 bp), one small single copy (SSC) region (19,047 bp), and a pair of inverted repeats (IR) (25,449 bp each). The GC content of this genome was 35.9%. The whole genome contained 136 genes, including 88 protein-coding genes, 40 tRNA genes, and eight rRNA genes. Phylogenetic analysis indicated that R. sinensis formed a distinct clade, being separated from Zostera marina and Potamogeton perfoliatus.

Keywords: Illumina sequencing; Ruppia sinensis; chloroplast genome; phylogenetic position.