β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1 ) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

Dina N Abdelrahman; Aya A Taha; Mazar M Dafaallah; Alaa Abdelgafoor Mohammed; Abdel Rahim M El Hussein; Ahmed I Hashim; Yousif F Hamedelnil; Hisham N Altayb

doi:10.12688/f1000research.24818.3

β-lactamases (bla _TEM, bla _SHV, bla _CTXM-1, bla _VEB, bla _OXA-1 ) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

F1000Res. 2020 Jul 27:9:774. doi: 10.12688/f1000research.24818.3. eCollection 2020.

Authors

Dina N Abdelrahman^{1

2}, Aya A Taha², Mazar M Dafaallah², Alaa Abdelgafoor Mohammed³, Abdel Rahim M El Hussein¹, Ahmed I Hashim², Yousif F Hamedelnil², Hisham N Altayb⁴

Affiliations

¹ Department of Virology, Central Laboratory, Khartoum, Sudan.
² Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan.
³ Department of Pharmaceutical Biotechnology, College of Pharmacy, Ahfad University for Women, Omdurman, Khartoum, Sudan.
⁴ Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Background:Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps.aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes ( blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant to Imipenem while all isolates were resistant to Amoxicillin and Amoxyclav (100%). A total of 43 (54%) Ps. aeruginosa isolates were positive for blaTEM, blaSHV, blaCTXM-1, blaVEB and blaOXA-1 genes, while 27 (34%) were positive for class C β- Lactamases, and 20 (25%) were positive for both classes. Frequency of beta-lactamases genes was as follows: blaTEM, 19 (44.2%); blaSHV, 16 (37.2%); bla CTX-M1, 10 (23.3%); blaVEB, 14 (32.6%); blaOXA-1, 7 (16.3%). blaAmpC 22 (81.5%) and bla DHA 8 (29.6%). In total, 3 (11.1%) isolates were positive for both bla AmpC and blaDHA genes. Conclusion:Ps. aeruginosa isolates showed a high rate of β- lactamases production, with co-resistance to other antibiotic classes. The lowest resistance rate of Ps. aeruginosa was to Imipenem followed by Gentamicin and Ciprofloxacin. No statistically significant relationship between production of β-lactamases in Ps. aeruginosa and resistance to third generation cephalosporins was found.

Keywords: ESBLs; Khartoum-Sudan.; Polymerase Chain Reaction; Ps. aeruginosa; class C β-lactamase; pyocyanin pigment.

MeSH terms

Cross-Sectional Studies
Gene Frequency
Microbial Sensitivity Tests
Pseudomonas aeruginosa* / genetics
Sudan
beta-Lactamases* / genetics

Substances

beta-Lactamases

Associated data

figshare/10.6084/m9.figshare.12453287.v2

Grants and funding

The author(s) declared that no grants were involved in supporting this work.