Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

Jianping Jiang; Xiang Yuan; Guanghua Huang; Wen Shi; Xueming Yang; Qinyang Jiang; Yinhai Jia; Xiurong Yang; Hesheng Jiang

doi:10.1371/journal.pone.0243778

Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

PLoS One. 2020 Dec 23;15(12):e0243778. doi: 10.1371/journal.pone.0243778. eCollection 2020.

Authors

Jianping Jiang^{1

2}, Xiang Yuan^{2

3}, Guanghua Huang⁴, Wen Shi^{2

3}, Xueming Yang⁴, Qinyang Jiang², Yinhai Jia⁵, Xiurong Yang², Hesheng Jiang²

Affiliations

¹ Guangxi Engineering Technology Research Center of Chinese Medicinal Materials Stock Breeding, Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, China.
² College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.
³ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi, China.
⁴ Guangxi Academy of Fisheries Sciences, Nanning, Guangxi, China.
⁵ Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

Abstract

The giant freshwater prawn, Macrobrachium rosenbergii (M. rosenbergii) as an important freshwater aquaculture species with high commercial value, exhibited unsynchronized growth. However, the potentially metabolic mechanism remains unclear. In this study, we used liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the hepatopancreatic metabolic profiles of twenty giant freshwater prawns between the fast-growing group and slow-growing group. In the metabolomics assay, we isolated 8,293 peaks in positive and negative iron mode. Subsequently, 44 significantly differential metabolites were identified. Functional pathway analysis revealed that these metabolites were significantly enriched in three key metabolic pathways. Further integrated analysis indicated that glycerophospholipid metabolism and aminoacyl-tRNA biosynthesis have significant impact on growth performance in M.rosenbergii. Our findings presented here demonstrated the critical metabolites and metabolic pathways involved in growth performance, moreover provided strong evidence for elucidating the potentially metabolic mechanism of the unsynchronized growth in M. rosenbergii.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bile Ducts*
Metabolomics*
Palaemonidae / growth & development*
Palaemonidae / metabolism*
Pancreas*

Grants and funding

This work was financially supported by the Natural Science Foundation of Guangxi, grant number 2018GXNSFBA281209; Science and Technology Major Project of Guangxi, grant number Guike AA17204080-6; and Postdoctoral Science Foundation of Guangxi, grant number T3340097968. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.