Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

Axel J Chu; Chinten James Lim

doi:10.1007/7651_2020_335

Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

Methods Mol Biol. 2022:2454:117-126. doi: 10.1007/7651_2020_335.

Authors

Axel J Chu¹, Chinten James Lim²

Affiliations

¹ University of British Columbia, Vancouver, BC, Canada.
² Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. cjlim@mail.ubc.ca.

PMID: 33349904
DOI: 10.1007/7651_2020_335

Abstract

Various types of stem cells and nonstem cells have been shown to differentiate or transdifferentiate into cardiomyocytes by way of coculture with appropriate inducer cells. Here we describe a method to induce cardiac differentiation in induced pluripotent stem (iPS) cells through the use of coculture with previously differentiated iPS cell-derived cardiomyocytes (iCMs). This differentiation process can be achieved without the use of exogenous pathway inhibitors and morphogens.

Keywords: Co-culture; Induced pluripotent stem cell-derived cardiomyocytes; Induced pluripotent stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Coculture Techniques
Induced Pluripotent Stem Cells*
Myocytes, Cardiac