Generation of a FOXH1 homozygous knockout human embryonic stem cell line by CRISPR/Cas9 system

Tian Zhang; Wanlan Huang; Xingkui Xue

doi:10.1016/j.scr.2020.102121

Generation of a FOXH1 homozygous knockout human embryonic stem cell line by CRISPR/Cas9 system

Stem Cell Res. 2020 Dec 10:50:102121. doi: 10.1016/j.scr.2020.102121. Online ahead of print.

Authors

Tian Zhang¹, Wanlan Huang², Xingkui Xue³

Affiliations

¹ Medical Research Center, People's Hospital of Longhua, Shenzhen 518109, China.
² Department of Oncology, Jieyang People's Hospital, Jieyang 522000, China.
³ Medical Research Center, People's Hospital of Longhua, Shenzhen 518109, China. Electronic address: xuexk@outlook.com.

PMID: 33348200
DOI: 10.1016/j.scr.2020.102121

Abstract

Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous knockout human embryonic stem cell (hESC), WAe009-A-42 by CRISPR/Cas9 mediated gene targeting. The WAe009-A-42 retained a typical undifferentiated morphology and normal karyotype, pluripotenty, and trilineage differentiation potential in vivo and vitro.