Bioprocessing of Chaetoceros calcitrans for the recovery of fucoxanthin using CO2-based alkyl carbamate ionic liquids

Kuan Shiong Khoo; Chien Wei Ooi; Kit Wayne Chew; Su Chern Foo; Pau Loke Show

doi:10.1016/j.biortech.2020.124520

Bioprocessing of Chaetoceros calcitrans for the recovery of fucoxanthin using CO₂-based alkyl carbamate ionic liquids

Bioresour Technol. 2021 Feb:322:124520. doi: 10.1016/j.biortech.2020.124520. Epub 2020 Dec 10.

Authors

Kuan Shiong Khoo¹, Chien Wei Ooi², Kit Wayne Chew³, Su Chern Foo⁴, Pau Loke Show⁵

Affiliations

¹ Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
² Chemical Engineering Discipline and Advanced Engineering Platform, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia. Electronic address: ooi.chien.wei@monash.edu.
³ School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor, Malaysia. Electronic address: kitwayne.chew@xmu.edu.my.
⁴ School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia. Electronic address: foo.suchern@monash.edu.
⁵ Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. Electronic address: PauLoke.Show@nottingham.edu.my.

PMID: 33348114
DOI: 10.1016/j.biortech.2020.124520

Abstract

Ionic liquids (ILs) have emerged as an alternative solvent used in the bioprocessing of microalgae for recovery of valuable biomolecules. The aim of this work is to extract fucoxanthin from Chaetoceros calcitrants (C. calcitrans) by using the readily distillable CO₂-based alkyl carbamate ILs. The degree of cell permeabilization was analysed by the quantification of extracted fucoxanthin and the analyses of cell surface morphology. Among the tested CO₂-based alkyl carbamate ILs, diallylammonium diallylcarbamate (DACARB) extraction system gave the maximal yield of fucoxanthin at 17.51 mg/g under the optimal extraction conditions [90% (v/v), 3 min and 25 °C]. Moreover, the extracted fucoxanthin fraction exhibited the satisfactory antioxidant activities. The recyclability of DACARB was demonstrated in the multiple batches of fucoxanthin extraction. Hence, CO₂-based alkyl carbamate ILs can prospectively substitute conventional organic solvents in the downstream processing of bioactive compounds from microalgae.

Keywords: CO(2); Carotenoids; Chaetoceros calcitrans; Extraction; Fucoxanthin; Ionic liquids.

MeSH terms

Carbamates
Carbon Dioxide
Ionic Liquids*
Xanthophylls

Substances

Carbamates
Ionic Liquids
Xanthophylls
fucoxanthin
Carbon Dioxide