Identification of microRNA-like RNAs in Cordyceps guangdongensis and their expression profile under differential developmental stages

Gangzheng Wang; Min Li; Chenghua Zhang; Ning Zhan; Huijiao Cheng; Yu Gao; Chengyuan Sun; Wangqiu Deng; Taihui Li

doi:10.1016/j.fgb.2020.103505

Identification of microRNA-like RNAs in Cordyceps guangdongensis and their expression profile under differential developmental stages

Fungal Genet Biol. 2021 Feb:147:103505. doi: 10.1016/j.fgb.2020.103505. Epub 2020 Dec 30.

Authors

Gangzheng Wang¹, Min Li², Chenghua Zhang¹, Ning Zhan³, Huijiao Cheng⁴, Yu Gao³, Chengyuan Sun⁴, Wangqiu Deng⁵, Taihui Li⁶

Affiliations

¹ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
² Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; College of Agriculture and Animal Husbandry, Tibet University, Nyingchi 860000, China.
³ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
⁴ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; South China Agricultural University, Guangzhou 510642, China.
⁵ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China. Electronic address: dengwq@gdim.cn.
⁶ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China. Electronic address: lith@gdim.cn.

PMID: 33347973
DOI: 10.1016/j.fgb.2020.103505

Abstract

Cordyceps guangdongensis is a well-known fungus with high nutritional and medicinal value. The metabolite profile of C. guangdongensis is similar to that of Ophiocordyceps sinensis. In plants and animals, microRNAs play important roles in regulating gene expression at the post-transcriptional level. MicroRNA-like RNAs (milRNAs) have been documented in several macro-fungi. To comprehensively investigate the milRNAs in C. guangdongensis, three small RNA libraries from the differentially developmental stages were constructed. Twenty-six conserved milRNAs were identified, and 19 novel milRNA candidates were predicted. Among them, 20 milRNAs were differentially expressed across the developmental processes, and 12 milRNAs were verified using stem-loop quantitative real-time reverse transcription polymerase chain reaction. In addition, the potential target genes of milRNA were predicted to be involved in the development of fruiting bodies and metabolite biosynthesis. This study is the first to report the milRNAs of C. guangdongensis, and provides important insights into studies of milRNA regulation pathways in ascomycete fungi.

Keywords: Cordyceps; Small RNA sequencing; Stem-loop qRT-PCR; microRNA-like RNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cordyceps / genetics*
Cordyceps / growth & development*
Gene Expression Profiling
Gene Expression Regulation, Fungal*
High-Throughput Nucleotide Sequencing
Metabolic Networks and Pathways / genetics
MicroRNAs / genetics*
MicroRNAs / isolation & purification
RNA, Fungal / genetics*

Substances

MicroRNAs
RNA, Fungal

Supplementary concepts

Cordyceps guangdongensis