Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors

Svitlana Vasylovska; Jens Schuster; Anja Brboric; Per-Ola Carlsson; Niklas Dahl; Joey Lau

doi:10.1016/j.scr.2020.102114

Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors

Stem Cell Res. 2020 Dec 9:50:102114. doi: 10.1016/j.scr.2020.102114. Online ahead of print.

Authors

Svitlana Vasylovska¹, Jens Schuster², Anja Brboric¹, Per-Ola Carlsson¹, Niklas Dahl², Joey Lau³

Affiliations

¹ Uppsala University, Department of Medical Cell Biology, Uppsala, Sweden.
² Uppsala University, Department of Immunology, Genetics and Pathology and Science for Life Laboratory, Uppsala, Sweden.
³ Uppsala University, Department of Medical Cell Biology, Uppsala, Sweden. Electronic address: Joey.Lau@mcb.uu.se.

PMID: 33340796
DOI: 10.1016/j.scr.2020.102114

Abstract

Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening.