Field work was performed to investigate the release of hydrogen sulphide (H2S) and its transport in the sewer trunk with drops in the Bonnie Doon area in Edmonton, Alberta, Canada, in order to develop a proper odor control strategy. The liquid sulfide concentration in the upstream trunk was low (less than 1.0 mg/L), and no H2S gas was detected in the head space under this low concentration. However, high H2S gas concentration was detected in the middle reach of the trunk due to the stripping effect of the three drops (2.7 m, 5.2 m and 2.0 m) along the trunk. The released H2S at drops was then transported in the sewer system and emitted at various locations and caused odor concerns. These drops played an important role in H2S release, and the overall H2S mass transfer coefficient at drops was much higher than that in normal gravity sewers. The overall oxygen and H2S mass transfer coefficient (KLa) was estimated to be around 200 h-1 and 300 h-1 at the first two drops, respectively. Field sampling of biofilm indicates that Desulfomicrobium was identified as the sulfate-reducing bacteria (SRB) responsible for sulfide generation in sewer wall biofilm and Thiobacillus was the only predominant member in manhole wall biofilm contributing to sewer manhole corrosion.