Stabilization of maneb group by ethylenediamine and direct-determination by liquid chromatography tandem mass spectrometry

Yuanjia Chen; Zhuofeng Ke; Zhenlin Xu; Weilong Huang; Yuanming Sun; Hongtao Lei; Xiaoqun Wei

doi:10.1016/j.foodchem.2020.128774

Stabilization of maneb group by ethylenediamine and direct-determination by liquid chromatography tandem mass spectrometry

Food Chem. 2021 May 30:345:128774. doi: 10.1016/j.foodchem.2020.128774. Epub 2020 Dec 3.

Authors

Yuanjia Chen¹, Zhuofeng Ke², Zhenlin Xu³, Weilong Huang⁴, Yuanming Sun⁵, Hongtao Lei⁶, Xiaoqun Wei⁷

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: chenyuanjia@stu.scau.edu.cn.
² School of Materials Science & Engineering, PCFM Lab, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: kezhf3@mail.sysu.edu.cn.
³ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: jallent@163.com.
⁴ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: 2857649104@qq.com.
⁵ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: ymsun@scau.edu.cn.
⁶ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: hongtao@scau.edu.cn.
⁷ Guangdong Provincial Key Laboratory of Food Quality and Safety/ Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: weixqun@scau.edu.cn.

PMID: 33338835
DOI: 10.1016/j.foodchem.2020.128774

Abstract

A rapid, low-cost, and selective method for simultaneous and direct determination of maneb group residues (containing ethylenebis and propylenebis dithiocarbamates) in fruit by liquid chromatography coupled to tandem mass spectrometry was developed and validated in the current study. The results showed the maneb group could be melt and stabilized by 5 v% ethylenediamine for 60 days keeping in conventional refrigerators, in which a stable and ionizable pentadentate ligand complex was considered to be formed by the bidentate diamine and sulfhydryl followed by Density Functional Theory calculation. The validated method showed a sensitive quantification limits (0.03 mg/kg), a steady recovery (82.1%-91.0%) and an excellent precision (2.7%-4.3% RSD). This method is applied to analyze fruit samples and achieved satisfactory results. Therefore, this method can be proposed as a robust analytical method of maneb group in fruit, and can be adapted to detect other compounds with sulfhydryl group.

Keywords: Direct-determination; High performance liquid chromatography-tandem mass spectrometry; Maneb group; Stabilization.

MeSH terms

Chromatography, Liquid / methods*
Ethylenediamines / chemistry*
Fruit / chemistry
Maneb / analysis*
Maneb / chemistry*
Tandem Mass Spectrometry / methods*

Substances

Ethylenediamines
Maneb