An Omalizumab Biobetter Antibody With Improved Stability and Efficacy for the Treatment of Allergic Diseases

Front Immunol. 2020 Nov 27:11:596908. doi: 10.3389/fimmu.2020.596908. eCollection 2020.

Abstract

The critical role of IgE in allergic diseases is well-documented and clinically proven. Omalizumab, a humanized anti-IgE antibody, was the first approved antibody for the treatment of allergic diseases. Nevertheless, omalizumab still has some limitations, such as product instability and dosage restriction in clinical application. In this study, we attempted to develop an omalizumab biobetter antibody with the potential to overcome its limitations. We removed two aspartic acid isomerization hotspots in CDRs of omalizumab to improve antibody candidate's stability. Meanwhile, several murine amino acids in the framework region of omalizumab were replaced with human source to reduce the potential immunogenicity. Yeast display technology was then applied to screen antibody candidates with high binding affinity to IgE. Moreover, YTE mutation in Fc fragment was introduced into the candidates for extending their serum half-life. A lead candidate, AB1904Am15, was screened out, which showed desired biophysical properties and improved stability, high binding affinity and elevated potency in vitro, prolonged half-life in human FcRn transgenic mouse, and enhanced in vivo efficacy in cynomolgus monkey asthma model. Overall, our study developed a biobetter antibody of omalizumab, AB1904Am15, which has the potential to show improved clinical benefit in the treatment of allergic diseases.

Keywords: IgE; affinity; allergic diseases; biobetter; efficacy; half-life; stability.

MeSH terms

  • Anti-Allergic Agents / chemistry
  • Anti-Allergic Agents / pharmacology*
  • Anti-Allergic Agents / therapeutic use*
  • Antibodies, Anti-Idiotypic / chemistry
  • Antibodies, Anti-Idiotypic / pharmacology*
  • Antibodies, Anti-Idiotypic / therapeutic use*
  • Antibody Affinity / immunology
  • Biophysical Phenomena
  • Chromatography, Liquid
  • Drug Monitoring
  • Drug Stability
  • Flow Cytometry
  • Humans
  • Hypersensitivity / diagnosis
  • Hypersensitivity / drug therapy*
  • Hypersensitivity / immunology
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Omalizumab / chemistry
  • Omalizumab / pharmacology*
  • Omalizumab / therapeutic use*
  • Protein Binding
  • Tandem Mass Spectrometry
  • Treatment Outcome

Substances

  • Anti-Allergic Agents
  • Antibodies, Anti-Idiotypic
  • anti-IgE antibodies
  • Omalizumab
  • Immunoglobulin E