Saline conditions effect on the performance and stress index of anaerobic ammonium oxidizing (anammox) bacteria

Victory Fiifi Dsane; Sumin An; Taeseok Oh; Jiyun Hwang; Yuri Choi; Younggyun Choi

doi:10.1016/j.chemosphere.2020.129227

Saline conditions effect on the performance and stress index of anaerobic ammonium oxidizing (anammox) bacteria

Chemosphere. 2021 Mar:267:129227. doi: 10.1016/j.chemosphere.2020.129227. Epub 2020 Dec 6.

Authors

Victory Fiifi Dsane¹, Sumin An², Taeseok Oh³, Jiyun Hwang², Yuri Choi², Younggyun Choi⁴

Affiliations

¹ Department of Environmental Engineering, Chungnam National University, Daejeon, South Korea; Department of Food Process Engineering, University of Ghana, Legon, Ghana.
² Department of Environmental Engineering, Chungnam National University, Daejeon, South Korea.
³ Department of Environmental Engineering, Chungnam National University, Daejeon, South Korea; BKT Company Ltd., Korea, Sinseong-dong, Daejeon, South Korea.
⁴ Department of Environmental Engineering, Chungnam National University, Daejeon, South Korea. Electronic address: youngchoi@cnu.ac.kr.

PMID: 33326902
DOI: 10.1016/j.chemosphere.2020.129227

Abstract

In this study, a lab-scale sequencing batch reactor dominated by freshwater anammox bacteria (FAB) was used to study the performance and stress index of the anammox bacteria at various saline conditions. The reactor with an effective volume of 1.8 L was operated for about 160 days. The nitrogen-loading rate was maintained at 0.364 kg-N m^-3d^-1 throughout the operational period. At the start-up phase, the seed biomass acclimation to the lab bioreactor showed an inconsistent performance. However, a stable performance was observed after day 38. The average substrate removal efficiency was 92% during most of the operational period. Anammox stress index; a ratio of dissolved Adenosine Triphosphate (dATP_amx) to total Adenosine Triphosphate (tATP_amx) showed an irrefutable correlation between NaCl concentration, anammox stress and microbial community. A drop in the biomass cellular ATP at 5 g L^-1 salinity led to a significant decrease in the Specific Anammox activity. Candidatus Brocadia was identified as the main anammox species and its relative abundance reduced along the stepwise salinity increment.

Keywords: Adenosine triphosphate (ATP); Anammox; Salinity; Sequencing batch reactor (SBR); Stress index.

MeSH terms

Ammonium Compounds*
Anaerobiosis
Bacteria / genetics
Bacteria, Anaerobic
Bioreactors
Nitrogen / analysis
Oxidation-Reduction
Sewage

Substances

Ammonium Compounds
Sewage
Nitrogen