Particulate Matter Exposure During Oocyte Maturation: Cell Cycle Arrest, ROS Generation, and Early Apoptosis in Mice

Abstract

Particulate matter (PM) is a general atmospheric pollutant released into the air by an anthropogenic and naturally derived mixture of substances. Current studies indicate that fine dust can result in different health defects, including endothelial dysfunction, asthma, lung cancer, cardiovascular diseases, uterine leiomyoma, deterioration in sperm quality, and overall birth impairment. However, the most prominent effects of PM₁₀ (diameter < 10 μM) exposure on the female reproductive system, especially with respect to oocyte maturation, remain unclear. In the present study, maturing mouse oocytes were treated with PM₁₀ and the phenotypes of the resulting toxic effects were investigated. Exposure to PM₁₀ led to impairment of maturation capacity by inducing cell cycle arrest and blocking normal polar body extrusion during in vitro maturation and activation of fertilization of mouse oocytes. Additionally, defects in tubulin formation and DNA alignment were observed in PM₁₀-treated oocytes during metaphase I to anaphase/telophase I transition. Moreover, PM₁₀ induced reactive oxygen species generation, mitochondrial dysfunction, DNA damage, and early apoptosis. Taken together, these results indicate that PM₁₀ exposure leads to a decline in oocyte quality and affects the subsequent embryonic development potential of mammalian oocytes.

Keywords: cell cycle arrest; oocyte maturation; particulate matter; polar body extrusion; time-lapse microscopy.