Rutin-Loaded Silver Nanoparticles With Antithrombotic Function

Haitao Wu; Manlin Su; Hui Jin; Xinyu Li; Puyu Wang; Jingxiao Chen; Jinghua Chen

doi:10.3389/fbioe.2020.598977

Rutin-Loaded Silver Nanoparticles With Antithrombotic Function

Front Bioeng Biotechnol. 2020 Nov 25:8:598977. doi: 10.3389/fbioe.2020.598977. eCollection 2020.

Authors

Haitao Wu¹, Manlin Su¹, Hui Jin¹, Xinyu Li¹, Puyu Wang¹, Jingxiao Chen¹, Jinghua Chen¹

Affiliation

¹ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China.

Abstract

In this paper, we fabricated rutin-loaded silver nanoparticles (Rutin@AgNPs) as the nano-anticoagulant with antithrombotic function. The serum stability, anticoagulation activity, and bleeding risk of Rutin@AgNPs were evaluated. The results showed Rutin@AgNPs had good serum stability, hemocompatibility, and cytocompatibility. The anticoagulation activity of rutin was maintained, and its stability and aqueous solubility were improved. The Rutin@AgNPs could provide a sustained release to prolong the half-life of rutin. The results of the coagulation parameter assay and thrombus formation test in mice model showed that the activated partial thromboplastin time and prothrombin time were prolonged, and Rutin@AgNPs inhibited the thrombosis in the 48 h period. Moreover, the limited bleeding time indicated that the Rutin@AgNPs significantly minimized the hemorrhage risk of rutin. This Rutin@AgNPs is a potential anticoagulant for antithrombotic therapy.

Keywords: anticoagulant; antithrombosis; rutin; silver nanoparticles; sustained release.